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The Enzyme Conservation Law states that the total enzyme concentration remains constant throughout the reaction. The initial enzyme concentration equals the sum of free enzyme, enzyme-substrate complex, and enzyme-inhibitor complex concentrations.
The calculator uses the enzyme conservation equation:
Where:
Explanation: This equation calculates the concentration of enzyme-inhibitor complex by subtracting free enzyme and enzyme-substrate complex concentrations from the total initial enzyme concentration.
Details: Calculating enzyme-inhibitor complex concentration is crucial for understanding enzyme kinetics, studying inhibition mechanisms, and determining inhibitor potency in biochemical reactions.
Tips: Enter initial enzyme concentration, catalyst concentration, and enzyme-substrate complex concentration in mol/m³. All values must be non-negative and consistent with the conservation law.
Q1: What is enzyme inhibition?
A: Enzyme inhibition occurs when a molecule binds to an enzyme and decreases its activity, which can be competitive, non-competitive, or uncompetitive.
Q2: Why is enzyme conservation important?
A: The enzyme conservation law ensures mass balance in enzymatic reactions and is fundamental for deriving kinetic equations and analyzing enzyme behavior.
Q3: What units should be used for concentration values?
A: Concentrations should be in consistent units (mol/m³ recommended). Ensure all measurements are in the same units for accurate calculations.
Q4: Can this calculator handle negative results?
A: Negative results indicate inconsistent input values that violate the enzyme conservation law. Please verify your measurements.
Q5: What if I have multiple inhibitors?
A: This calculator assumes single inhibitor conditions. For multiple inhibitors, more complex equations considering different inhibition types are needed.