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The Enzyme Conservation Law states that the total enzyme concentration remains constant throughout the reaction. In the presence of an inhibitor, the enzyme can exist in three forms: free enzyme (E), enzyme-substrate complex (ES), and enzyme-inhibitor complex (EI).
The calculator uses the enzyme conservation formula:
Where:
Explanation: This formula calculates the concentration of enzyme-substrate complex by subtracting the concentrations of free enzyme and enzyme-inhibitor complex from the total initial enzyme concentration.
Details: Calculating the enzyme-substrate complex concentration is crucial for understanding enzyme kinetics, determining reaction rates, and studying the effects of inhibitors on enzymatic reactions.
Tips: Enter all concentrations in mol/m³. Ensure values are non-negative and the sum of E and EI does not exceed the initial enzyme concentration for meaningful results.
Q1: What is enzyme conservation law?
A: The enzyme conservation law states that the total amount of enzyme remains constant throughout the reaction, distributed among free enzyme, enzyme-substrate complex, and enzyme-inhibitor complex.
Q2: Why is this calculation important?
A: This calculation helps determine the concentration of the active enzyme-substrate complex, which is essential for understanding reaction kinetics and inhibitor effects.
Q3: What units should be used for concentration values?
A: All concentrations should be in consistent units, typically mol/m³ or mol/L. The calculator uses mol/m³ as the standard unit.
Q4: What if the result is negative?
A: A negative result indicates that the sum of free enzyme and enzyme-inhibitor complex concentrations exceeds the initial enzyme concentration, which is not physically possible. Check your input values.
Q5: Can this calculator be used for different types of inhibitors?
A: Yes, this calculation applies to various types of enzyme inhibitors as it's based on the fundamental enzyme conservation principle.