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Definition: This calculator determines the adjusted retention time of the first component based on the adjusted retention time of a second component and their relative retention.
Purpose: It helps in chromatographic analysis to understand the separation behavior of different components in a mixture.
The calculator uses the formula:
Where:
Explanation: The adjusted retention time of the second component is divided by the relative retention to obtain the adjusted retention time of the first component.
Details: Adjusted retention time helps in identifying compounds and understanding their behavior in chromatographic separation by eliminating the void time of the column.
Tips: Enter the adjusted retention time of solute 2 in seconds and the relative retention (α). Both values must be > 0.
Q1: What is adjusted retention time?
A: It's the retention time minus the void time (time taken by unretained solute to travel through the column).
Q2: What does relative retention (α) represent?
A: It's the ratio of adjusted retention times of two components, indicating their separation.
Q3: How is relative retention determined?
A: It's calculated experimentally by dividing the adjusted retention times of two peaks in a chromatogram.
Q4: What if I get a negative result?
A: Negative values are impossible - check your inputs as both values must be positive.
Q5: How precise should my inputs be?
A: Use at least 3 decimal places for accurate results in chromatographic analysis.