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Definition: This calculator determines the adjusted retention time of the second component in chromatography based on relative retention and the first component's adjusted retention time.
Purpose: It helps in chromatographic analysis to predict retention behavior of compounds relative to each other.
The calculator uses the formula:
Where:
Explanation: The adjusted retention time of the second component is calculated by multiplying the relative retention factor by the adjusted retention time of the first component.
Details: Adjusted retention time is crucial in chromatography for compound identification, method development, and separation optimization.
Tips: Enter the relative retention (α) and the adjusted retention time of the first component. Both values must be > 0.
Q1: What is adjusted retention time?
A: It's the retention time minus the void time (time for unretained compound to pass through the column).
Q2: How is relative retention determined?
A: Relative retention (α) is the ratio of adjusted retention times of two components (always ≥ 1).
Q3: Why use adjusted retention instead of retention time?
A: Adjusted retention removes the mobile phase travel time, making comparisons more accurate.
Q4: What affects relative retention values?
A: Stationary phase chemistry, temperature, and mobile phase composition primarily affect α values.
Q5: Can this be used for HPLC and GC?
A: Yes, this calculation applies to all chromatographic techniques including HPLC, GC, and TLC.