Capacity Factor Formula:
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Definition: The capacity factor (k') is a measure of how long a component is retained by the chromatography column relative to the mobile phase.
Purpose: It helps in characterizing the retention behavior of analytes in chromatographic separations.
The calculator uses the formula:
Where:
Explanation: The formula relates the distribution of the analyte between the stationary and mobile phases.
Details: A higher capacity factor indicates stronger retention of the analyte by the stationary phase. Optimal values typically range between 1 and 10 for good separation.
Tips: Enter the concentrations and volumes for both phases. All values must be positive numbers. The result is dimensionless.
Q1: What does a capacity factor of 1 mean?
A: It means the analyte spends equal time in the stationary and mobile phases.
Q2: How does capacity factor relate to retention time?
A: The capacity factor is directly proportional to retention time. Higher k' means longer retention.
Q3: What's a typical range for capacity factor?
A: For good separation, k' values between 1 and 10 are typically desired.
Q4: Can capacity factor be less than 1?
A: Yes, but values <1 indicate weak retention and poor separation.
Q5: How does temperature affect capacity factor?
A: Increasing temperature generally decreases k' as analytes spend less time in the stationary phase.