Formula Used:
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Definition: This calculator determines the difference in retention times between two peaks in chromatography based on the resolution and average peak width.
Purpose: It helps analytical chemists understand the separation efficiency between components in chromatographic analysis.
The calculator uses the formula:
Where:
Explanation: The resolution multiplied by the average peak width gives the difference in retention times between two adjacent peaks.
Details: Understanding retention time differences helps optimize chromatographic conditions for better separation of compounds.
Tips: Enter the resolution (typically values between 1.0-2.0 for baseline separation) and average peak width in minutes. All values must be > 0.
Q1: What is a good resolution value?
A: A resolution of 1.5 or higher typically indicates baseline separation between peaks.
Q2: How do I measure peak width?
A: Peak width is typically measured at the baseline between the tangents drawn to the sides of the peak.
Q3: What affects retention time differences?
A: Mobile phase composition, column temperature, flow rate, and stationary phase characteristics all influence retention times.
Q4: Can this be used for HPLC and GC?
A: Yes, this calculation applies to both high-performance liquid chromatography (HPLC) and gas chromatography (GC).
Q5: Why is average width used?
A: Using the average width accounts for any asymmetry between the two peaks being compared.