Enzyme Catalyst Concentration Formula:
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Definition: This calculator determines the concentration of free enzyme catalyst based on the enzyme conservation law in biochemical reactions.
Purpose: It helps biochemists and researchers understand how much free enzyme is available during enzymatic reactions.
The calculator uses the formula:
Where:
Explanation: The free enzyme concentration equals the total enzyme minus what's bound in enzyme-substrate complexes.
Details: Knowing free enzyme concentration is crucial for understanding reaction kinetics, enzyme efficiency, and inhibition studies.
Tips: Enter the initial enzyme concentration and the measured enzyme-substrate complex concentration. Both values must be ≥ 0.
Q1: What is the enzyme conservation law?
A: It states that the total enzyme concentration equals free enzyme plus enzyme bound in complexes.
Q2: How do I measure enzyme-substrate complex concentration?
A: Typically through spectroscopic methods or activity assays that distinguish bound from free enzyme.
Q3: Can the result be negative?
A: No, the calculator prevents negative values as they're not physically meaningful in this context.
Q4: What units should I use?
A: The calculator uses mol/m³, but you can use any consistent units as long as both inputs are in the same units.
Q5: How does this relate to Michaelis-Menten kinetics?
A: This calculation is fundamental to deriving the Michaelis-Menten equation for enzyme kinetics.