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Concentration of Enzyme Catalyst by Enzyme Conservation Law Calculator

Enzyme Catalyst Concentration Formula:

\[ E = [E_0] - [ES] \]

mol/m³
mol/m³

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1. What is Concentration of Enzyme Catalyst by Enzyme Conservation Law?

Definition: This calculator determines the concentration of free enzyme catalyst based on the enzyme conservation law in biochemical reactions.

Purpose: It helps biochemists and researchers understand how much free enzyme is available during enzymatic reactions.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ E = [E_0] - [ES] \]

Where:

Explanation: The free enzyme concentration equals the total enzyme minus what's bound in enzyme-substrate complexes.

3. Importance of Enzyme Catalyst Concentration

Details: Knowing free enzyme concentration is crucial for understanding reaction kinetics, enzyme efficiency, and inhibition studies.

4. Using the Calculator

Tips: Enter the initial enzyme concentration and the measured enzyme-substrate complex concentration. Both values must be ≥ 0.

5. Frequently Asked Questions (FAQ)

Q1: What is the enzyme conservation law?
A: It states that the total enzyme concentration equals free enzyme plus enzyme bound in complexes.

Q2: How do I measure enzyme-substrate complex concentration?
A: Typically through spectroscopic methods or activity assays that distinguish bound from free enzyme.

Q3: Can the result be negative?
A: No, the calculator prevents negative values as they're not physically meaningful in this context.

Q4: What units should I use?
A: The calculator uses mol/m³, but you can use any consistent units as long as both inputs are in the same units.

Q5: How does this relate to Michaelis-Menten kinetics?
A: This calculation is fundamental to deriving the Michaelis-Menten equation for enzyme kinetics.

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