Enzyme Substrate Complex Concentration Formula:
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Definition: The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Purpose: It helps biochemists and researchers understand enzyme kinetics and reaction mechanisms.
The calculator uses the formula:
Where:
Explanation: The complex concentration is calculated by subtracting the free enzyme concentration from the total initial enzyme concentration.
Details: Understanding complex concentration is crucial for studying enzyme kinetics, determining reaction rates, and analyzing enzyme inhibition.
Tips: Enter the initial enzyme concentration and catalyst concentration in mol/m³. All values must be > 0 and initial enzyme concentration should be ≥ catalyst concentration.
Q1: What is the enzyme conservation law?
A: It states that the total enzyme concentration equals the sum of free enzyme and enzyme bound in complexes.
Q2: What units should I use for concentration?
A: The calculator uses mol/m³, but you can convert from other units (1 M = 1000 mol/m³).
Q3: What if my catalyst concentration is higher than initial enzyme?
A: This would violate the enzyme conservation law. Check your measurements.
Q4: Does this account for multiple substrates?
A: No, this is for single substrate systems. More complex models are needed for multiple substrates.
Q5: How does temperature affect the calculation?
A: Temperature affects reaction rates but not the conservation law itself.