Concentration of Mobile Phase given Capacity Factor Calculator

Concentration of Mobile Phase Formula:

\[ C_m = \frac{V_s \times C_s}{V_{mobile\ phase} \times k'} \]

Volume of Stationary Phase:

m³

Concentration of Stationary Phase:

mol/m³

Volume of Mobile Phase:

m³

Capacity Factor:

Concentration of Mobile Phase (mol/m³):

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1. What is Concentration of Mobile Phase given Capacity Factor?

Definition: This calculator determines the concentration of the mobile phase in chromatography based on the stationary phase properties and capacity factor.

Purpose: It helps analytical chemists and researchers optimize chromatographic separations by understanding the relationship between phase concentrations.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ C_m = \frac{V_s \times C_s}{V_{mobile\ phase} \times k'} \]

Where:

\( C_m \) — Concentration of mobile phase (mol/m³)
\( V_s \) — Volume of stationary phase (m³)
\( C_s \) — Concentration of stationary phase (mol/m³)
\( V_{mobile\ phase} \) — Volume of mobile phase (m³)
\( k' \) — Capacity factor (dimensionless)

Explanation: The formula relates the concentrations of both phases through their volumes and the capacity factor, which describes the retention of compounds.

3. Importance of Mobile Phase Concentration

Details: Proper mobile phase concentration is crucial for achieving optimal separation, resolution, and analysis time in chromatographic methods.

4. Using the Calculator

Tips: Enter the volume and concentration of stationary phase, volume of mobile phase, and capacity factor. All values must be > 0.

5. Frequently Asked Questions (FAQ)

Q1: What is the capacity factor (k')?
A: The capacity factor is a measure of how long a compound is retained on the column compared to an unretained compound.

Q2: What are typical values for stationary phase concentration?
A: This varies widely depending on the chromatography type, but 10,000 mol/m³ is common for many applications.

Q3: How does mobile phase concentration affect separation?
A: Higher concentrations typically lead to stronger elution power and shorter retention times.

Q4: What units should I use for volumes?
A: The calculator uses cubic meters (m³), but you can use any consistent volume units as long as they match.

Q5: Can this be used for all chromatography types?
A: This formula is most applicable to partition chromatography; other types may require modifications.