Concentration of Stationary Phase given Capacity Factor Calculator

Formula Used:

\[ C_s = \frac{C_m \times V_{mobile\ phase} \times k'}{V_s} \]

Concentration of Mobile Phase:

mol/m³

Volume of Mobile Phase:

m³

Capacity Factor:

Volume of Stationary Phase:

m³

Concentration of Stationary Phase:

mol/m³

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1. What is Concentration of Stationary Phase Calculator?

Definition: This calculator determines the concentration of the stationary phase in chromatography based on mobile phase properties and capacity factor.

Purpose: It helps analytical chemists and researchers understand the distribution of analytes between mobile and stationary phases in chromatographic separations.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ C_s = \frac{C_m \times V_{mobile\ phase} \times k'}{V_s} \]

Where:

\( C_s \) — Concentration of stationary phase (mol/m³)
\( C_m \) — Concentration of mobile phase (mol/m³)
\( V_{mobile\ phase} \) — Volume of mobile phase (m³)
\( k' \) — Capacity factor (dimensionless)
\( V_s \) — Volume of stationary phase (m³)

Explanation: The formula calculates how much of the analyte is retained in the stationary phase based on its interaction with both phases.

3. Importance of Stationary Phase Concentration

Details: Knowing the stationary phase concentration helps optimize chromatographic conditions, improve separation efficiency, and understand analyte-stationary phase interactions.

4. Using the Calculator

Tips: Enter all required parameters with positive values. Typical mobile phase concentrations range from 0.1-100 mol/m³, and capacity factors typically range from 1-10.

5. Frequently Asked Questions (FAQ)

Q1: What is the capacity factor (k')?
A: The capacity factor is a measure of how long a compound is retained on the column relative to an unretained compound.

Q2: How do I measure the volume of stationary phase?
A: It's typically determined by column specifications or through void volume measurements.

Q3: What units should I use for concentration?
A: The calculator uses mol/m³, but you can convert from other units (like mol/L) before entering values.

Q4: Can this be used for all chromatography types?
A: Yes, but interpretation may vary for different techniques (HPLC, GC, etc.).

Q5: What if I get an unrealistically high result?
A: Check your input values, especially the stationary phase volume, which is often much smaller than the mobile phase volume.