Enzyme Substrate Complex Concentration Formula:
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Definition: This calculator determines the concentration of the enzyme-substrate complex (ES) formed during enzyme-catalyzed reactions at instantaneous chemical equilibrium.
Purpose: It helps biochemists and researchers understand enzyme kinetics and reaction mechanisms by quantifying the intermediate complex formation.
The calculator uses the formula:
Where:
Explanation: The formula describes the equilibrium between enzyme-substrate complex formation and dissociation.
Details: Understanding ES complex concentration is crucial for determining reaction rates, enzyme efficiency, and inhibitor effects in biochemical systems.
Tips: Enter the forward rate constant, initial enzyme concentration, substrate concentration, and reverse rate constant. All values must be > 0.
Q1: What is the typical range for forward rate constants?
A: Forward rate constants typically range from 103 to 108 s-1 for enzyme-catalyzed reactions.
Q2: How does substrate concentration affect ES complex formation?
A: ES complex concentration increases with substrate concentration but eventually plateaus when all enzyme active sites are saturated.
Q3: What units should I use for concentration?
A: The calculator uses mol/m3 (equivalent to mM), but you can convert from other units before entering values.
Q4: How is this different from Michaelis-Menten kinetics?
A: This calculation assumes instantaneous equilibrium, while Michaelis-Menten considers steady-state conditions.
Q5: Can I use this for inhibitor studies?
A: Yes, but you'll need to adjust the rate constants to account for competitive or non-competitive inhibition.