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Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium Calculator

Enzyme Substrate Complex Concentration Formula:

\[ ES = \frac{k_f \times [E_0] \times S}{k_r + (k_f \times S)} \]

s-1
mol/m3
mol/m3
mol/m3·s

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1. What is Enzyme Substrate Complex Concentration?

Definition: This calculator determines the concentration of the enzyme-substrate complex (ES) formed during enzyme-catalyzed reactions at instantaneous chemical equilibrium.

Purpose: It helps biochemists and researchers understand enzyme kinetics and reaction mechanisms by quantifying the intermediate complex formation.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ ES = \frac{k_f \times [E_0] \times S}{k_r + (k_f \times S)} \]

Where:

Explanation: The formula describes the equilibrium between enzyme-substrate complex formation and dissociation.

3. Importance of ES Complex Calculation

Details: Understanding ES complex concentration is crucial for determining reaction rates, enzyme efficiency, and inhibitor effects in biochemical systems.

4. Using the Calculator

Tips: Enter the forward rate constant, initial enzyme concentration, substrate concentration, and reverse rate constant. All values must be > 0.

5. Frequently Asked Questions (FAQ)

Q1: What is the typical range for forward rate constants?
A: Forward rate constants typically range from 103 to 108 s-1 for enzyme-catalyzed reactions.

Q2: How does substrate concentration affect ES complex formation?
A: ES complex concentration increases with substrate concentration but eventually plateaus when all enzyme active sites are saturated.

Q3: What units should I use for concentration?
A: The calculator uses mol/m3 (equivalent to mM), but you can convert from other units before entering values.

Q4: How is this different from Michaelis-Menten kinetics?
A: This calculation assumes instantaneous equilibrium, while Michaelis-Menten considers steady-state conditions.

Q5: Can I use this for inhibitor studies?
A: Yes, but you'll need to adjust the rate constants to account for competitive or non-competitive inhibition.

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