Enzyme Substrate Dissociation Constant Formula:
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Definition: The Enzyme Substrate Dissociation Constant (Ki') represents the affinity between an enzyme and its substrate when modified by an inhibitor.
Purpose: This calculator helps biochemists determine the dissociation constant based on inhibitor concentration and the enzyme substrate modifying factor.
The calculator uses the formula:
Where:
Explanation: The inhibitor concentration is divided by (modifying factor - 1) to determine the dissociation constant.
Details: This constant is crucial for understanding enzyme kinetics, inhibitor effects, and designing enzyme-based therapies or industrial processes.
Tips: Enter the inhibitor concentration in mol/m³ and the enzyme substrate modifying factor (must be > 1 for valid calculation).
Q1: What does a high Ki' value indicate?
A: A high value suggests weak binding between the enzyme and substrate when modified by the inhibitor.
Q2: What's a typical range for the modifying factor?
A: The modifying factor typically ranges from 1.1 to 10, depending on the inhibitor's strength.
Q3: Why must the modifying factor be greater than 1?
A: Values ≤ 1 would give negative or infinite results, which are physically meaningless in this context.
Q4: How is inhibitor concentration typically determined?
A: Through experimental measurements like spectrophotometry or titration.
Q5: Can this calculator be used for competitive inhibition?
A: This specific formula applies to uncompetitive inhibition. Different formulas apply for competitive inhibition.