Enzyme Substrate Inhibitor Complex Formula:
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Definition: The Enzyme Substrate Inhibitor (ESI) Complex Concentration is the molar concentration of the complex formed when an uncompetitive inhibitor binds to the enzyme-substrate complex.
Purpose: This calculation helps biochemists and pharmaceutical researchers understand enzyme kinetics in the presence of inhibitors.
The calculator uses the formula:
Where:
Explanation: The concentration of ESI complex is directly proportional to both ES complex and inhibitor concentrations, and inversely proportional to the dissociation constant.
Details: Understanding ESI complex formation helps in drug design, enzyme inhibition studies, and biochemical pathway analysis.
Tips: Enter the enzyme-substrate complex concentration, inhibitor concentration, and dissociation constant. All values must be > 0.
Q1: What is an uncompetitive inhibitor?
A: An uncompetitive inhibitor binds only to the enzyme-substrate complex, not to the free enzyme.
Q2: How is Ki' different from Ki?
A: Ki' is the dissociation constant for the ESI complex, while Ki is for the EI complex in competitive inhibition.
Q3: What units should I use?
A: The calculator uses mol/m³, but you can use any consistent units as long as all inputs use the same units.
Q4: How do I determine the ES complex concentration?
A: ES concentration can be measured experimentally or calculated from enzyme kinetics equations.
Q5: What affects the dissociation constant?
A: Temperature, pH, and the specific enzyme-inhibitor interaction all influence Ki'.