Enzyme Substrate Modifying Factor Formula:
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Definition: The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
Purpose: It helps quantify how much an inhibitor affects the enzyme-substrate interaction in biochemical reactions.
The calculator uses the formula:
Where:
Explanation: The factor increases with higher inhibitor concentration and decreases with stronger enzyme-substrate binding (higher dissociation constant).
Details: This factor is crucial for understanding enzyme kinetics in the presence of inhibitors, which is important in drug development and biochemical research.
Tips: Enter the inhibitor concentration and enzyme-substrate dissociation constant in mol/m³. Both values must be > 0.
Q1: What does a modifying factor of 1 mean?
A: A factor of 1 means no inhibition is occurring (either no inhibitor present or it doesn't affect the enzyme-substrate complex).
Q2: How is the dissociation constant determined?
A: It's typically determined experimentally through kinetic studies, though it's difficult to measure directly since the enzyme-substrate complex is short-lived.
Q3: What's a typical range for this factor?
A: The factor typically ranges from 1 (no inhibition) up to 10 or more for strong inhibition scenarios.
Q4: Does this apply to all types of enzyme inhibition?
A: This specific formula applies to competitive inhibition. Other inhibition types have different modifying factors.
Q5: What units should I use for concentration?
A: The calculator uses mol/m³, but you can use any consistent units as long as both concentrations are in the same units.