1. What is Enzyme Substrate Complex Concentration in Presence of Uncompetitive Inhibitor?

Definition: This calculator determines the concentration of the enzyme-substrate complex (ES) when an uncompetitive inhibitor is present in the system.

Purpose: It helps biochemists and researchers understand enzyme kinetics and inhibition mechanisms in biochemical reactions.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( ES \) — Enzyme Substrate Complex Concentration (mol/m³)
• \( Ki' \) — Enzyme Substrate Dissociation Constant (mol/m³)
• \( ESI \) — Enzyme Substrate Inhibitor Complex Concentration (mol/m³)
• \( I \) — Inhibitor Concentration (mol/m³)

Explanation: The formula calculates the concentration of the enzyme-substrate complex by relating it to the inhibitor concentration and the dissociation constant.

3. Importance of This Calculation

Details: Understanding enzyme-substrate complex concentration helps in drug design, metabolic pathway analysis, and enzyme inhibition studies.

4. Using the Calculator

Tips: Enter the dissociation constant (Ki'), enzyme-substrate-inhibitor complex concentration (ESI), and inhibitor concentration (I). All values must be > 0.

5. Frequently Asked Questions (FAQ)

Q1: What is an uncompetitive inhibitor?
A: An uncompetitive inhibitor binds only to the enzyme-substrate complex, not to the free enzyme.

Q2: How does Ki' differ from Km?
A: Ki' is the dissociation constant for the enzyme-substrate-inhibitor complex, while Km is the Michaelis constant.

Q3: What units should I use for concentration?
A: The calculator uses mol/m³, but you can convert from other units (1 M = 1000 mol/m³).

Q4: Can this be used for competitive inhibition?
A: No, this formula is specific for uncompetitive inhibition models.

Q5: What if I get negative values?
A: All input values must be positive. Negative concentrations are not physically meaningful.