Stern Volmer Equation:
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Definition: The Stern Volmer equation describes the quenching of fluorescence intensity in the presence of a quencher molecule.
Purpose: It helps quantify how much a fluorescent signal decreases when a quencher is present, important in photochemistry and molecular spectroscopy.
The calculator uses the Stern Volmer equation:
Where:
Explanation: The equation shows that fluorescence intensity decreases with increasing quencher concentration, quenching constant, and singlet lifetime.
Details: This analysis helps determine quenching mechanisms, measure molecular interactions, and study excited state dynamics in photochemical systems.
Tips: Enter the initial intensity, singlet lifetime, quenching constant, and quencher concentration. All values must be > 0.
Q1: What is fluorescence quenching?
A: It's any process that decreases the fluorescence intensity of a substance, typically through molecular interactions.
Q2: What are typical values for the quenching constant?
A: Values range widely but are typically between 10⁶ to 10¹⁰ M⁻¹s⁻¹ depending on the quencher-fluorophore pair.
Q3: How do I measure singlet lifetime?
A: It's typically measured using time-resolved fluorescence spectroscopy techniques.
Q4: What are common quenchers?
A: Common quenchers include oxygen, halides, amines, and many organic molecules.
Q5: What does a linear Stern Volmer plot indicate?
A: A linear plot typically indicates a single, static quenching mechanism.