1. What is Fluoroscence Intensity?

Definition: Fluoroscence Intensity is defined as the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.

Purpose: This calculator helps determine the intensity of fluorescence emission based on the rate constants and absorption intensity.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( I_F \) — Fluoroscence Intensity (W/m²)
• \( K_f \) — Rate Constant of Fluoroscence (Hz)
• \( I_a \) — Absorption Intensity (W/m²)
• \( K_{NR} \) — Rate Constant of Non Radiative Reaction (Hz)

Explanation: The formula calculates the fluorescence intensity by considering both radiative and non-radiative decay processes.

3. Importance of Fluoroscence Intensity Calculation

Details: Accurate calculation of fluorescence intensity is crucial in spectroscopy, chemical analysis, and various scientific research applications.

4. Using the Calculator

Tips: Enter the rate constant of fluorescence, absorption intensity, and rate constant of non-radiative reaction. All values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: What affects fluorescence intensity?
A: Fluorescence intensity depends on the absorption intensity, quantum yield, and competition between radiative and non-radiative decay processes.

Q2: What are typical values for K_f?
A: Typical fluorescence rate constants range from 10⁶ to 10⁹ s^-1 depending on the fluorophore.

Q3: What does K_NR represent?
A: K_NR represents all non-radiative decay processes including internal conversion and intersystem crossing.

Q4: How is absorption intensity measured?
A: Absorption intensity is typically obtained by integrating the area under the absorption spectrum.

Q5: What if K_NR is zero?
A: If K_NR is zero, the fluorescence intensity equals the absorption intensity (I_F = I_a).