Fluorosence Intensity Formula:
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Definition: This calculator estimates the fluorescence intensity at low solute concentration based on quantum yield, initial intensity, molar extinction coefficient, concentration, and path length.
Purpose: It helps researchers and scientists determine fluorescence intensity in spectroscopic measurements.
The calculator uses the formula:
Where:
Explanation: The formula calculates fluorescence intensity by considering the quantum efficiency, excitation intensity, absorption characteristics, sample concentration, and path length.
Details: Accurate fluorescence intensity measurements are crucial for quantitative analysis in spectroscopy, chemical sensing, and biological imaging.
Tips: Enter all required parameters with appropriate units. Ensure all values are positive numbers for valid calculations.
Q1: What is typical quantum yield range?
A: Quantum yield typically ranges from 0 to 1, where 0 means no emission and 1 means every absorbed photon results in an emitted photon.
Q2: How does concentration affect the result?
A: At low concentrations, fluorescence intensity is directly proportional to concentration. At higher concentrations, nonlinear effects may occur.
Q3: What's a typical molar extinction coefficient?
A: This varies widely by molecule, but values often range from 10 to 100,000 m²/mol.
Q4: Why is path length important?
A: Longer path lengths allow more absorption of excitation light, typically leading to greater fluorescence intensity.
Q5: What units should I use?
A: Use consistent SI units as indicated for each input field (W/m² for intensity, m²/mol for extinction coefficient, etc.).