Forward Rate Constant Formula:
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Definition: This calculator determines the forward rate constant (kf) in enzyme kinetics based on the reverse rate constant, catalytic rate constant, and Michaelis constant.
Purpose: It helps biochemists and researchers understand enzyme kinetics and reaction rates in biological systems.
The calculator uses the formula:
Where:
Explanation: The sum of the reverse rate constant and catalytic rate constant is divided by the Michaelis constant to obtain the forward rate constant.
Details: Understanding the forward rate constant helps in studying enzyme kinetics, reaction mechanisms, and designing enzyme inhibitors for therapeutic purposes.
Tips: Enter the reverse rate constant (kr) in mol/m³·s, catalytic rate constant (kcat) in 1/s, and Michaelis constant (KM) in mol/m³. KM must be > 0.
Q1: What is the physical meaning of the forward rate constant?
A: It represents the rate at which the enzyme-substrate complex forms from the free enzyme and substrate.
Q2: How is the Michaelis constant (KM) determined experimentally?
A: KM is typically determined through kinetic experiments measuring reaction rates at varying substrate concentrations.
Q3: What units should I use for the input values?
A: Use mol/m³·s for kr, 1/s for kcat, and mol/m³ for KM to get kf in 1/s.
Q4: Can this calculator be used for non-enzymatic reactions?
A: The formula is specific to enzyme kinetics following Michaelis-Menten kinetics.
Q5: What's a typical range for forward rate constants in enzyme reactions?
A: They typically range from 10^4 to 10^8 M⁻¹s⁻¹ (or 10 to 10^5 m³/mol·s) for most enzymes.