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Forward Rate Constant given Michaelis Constant Calculator

Forward Rate Constant Formula:

\[ k_f = \frac{k_r + k_{cat}}{K_M} \]

mol/m³·s
1/s
mol/m³

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1. What is Forward Rate Constant given Michaelis Constant?

Definition: This calculator determines the forward rate constant (kf) in enzyme kinetics based on the reverse rate constant, catalytic rate constant, and Michaelis constant.

Purpose: It helps biochemists and researchers understand enzyme kinetics and reaction rates in biological systems.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ k_f = \frac{k_r + k_{cat}}{K_M} \]

Where:

Explanation: The sum of the reverse rate constant and catalytic rate constant is divided by the Michaelis constant to obtain the forward rate constant.

3. Importance of Forward Rate Constant Calculation

Details: Understanding the forward rate constant helps in studying enzyme kinetics, reaction mechanisms, and designing enzyme inhibitors for therapeutic purposes.

4. Using the Calculator

Tips: Enter the reverse rate constant (kr) in mol/m³·s, catalytic rate constant (kcat) in 1/s, and Michaelis constant (KM) in mol/m³. KM must be > 0.

5. Frequently Asked Questions (FAQ)

Q1: What is the physical meaning of the forward rate constant?
A: It represents the rate at which the enzyme-substrate complex forms from the free enzyme and substrate.

Q2: How is the Michaelis constant (KM) determined experimentally?
A: KM is typically determined through kinetic experiments measuring reaction rates at varying substrate concentrations.

Q3: What units should I use for the input values?
A: Use mol/m³·s for kr, 1/s for kcat, and mol/m³ for KM to get kf in 1/s.

Q4: Can this calculator be used for non-enzymatic reactions?
A: The formula is specific to enzyme kinetics following Michaelis-Menten kinetics.

Q5: What's a typical range for forward rate constants in enzyme reactions?
A: They typically range from 10^4 to 10^8 M⁻¹s⁻¹ (or 10 to 10^5 m³/mol·s) for most enzymes.

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