Forward Rate Constant Formula:
From: | To: |
Definition: The forward rate constant (kf) quantifies how quickly an enzyme binds to its substrate to form an enzyme-substrate complex.
Purpose: This calculator helps biochemists and enzymologists determine the rate constant for the forward reaction in enzyme kinetics.
The calculator uses the formula:
Where:
Explanation: The formula calculates the forward rate constant based on measurable quantities in enzyme kinetics experiments.
Details: Knowing kf helps understand enzyme efficiency, design inhibitors, and optimize industrial enzymatic processes.
Tips: Enter all required concentrations and the reverse rate constant. Ensure [E0] > [ES]. All values must be positive.
Q1: What are typical units for these constants?
A: kf is typically in s⁻¹, kr in mol/m³·s, and concentrations in mol/m³.
Q2: How do I measure [ES] experimentally?
A: [ES] can be estimated using spectroscopic methods or by measuring product formation rates.
Q3: What if [E0] ≤ [ES]?
A: This is physically impossible as you can't have more complex than initial enzyme.
Q4: How does temperature affect kf?
A: Like all rate constants, kf increases with temperature following Arrhenius equation.
Q5: Can I use this for non-enzymatic reactions?
A: The formula is specific for enzymatic mechanisms with ES complex formation.