Half Width of Peak Formula:
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Definition: This calculator determines the half-width of chromatographic peaks based on the number of theoretical plates and retention time.
Purpose: It helps in analyzing chromatographic column efficiency and peak characteristics in analytical chemistry.
The calculator uses the formula:
Where:
Explanation: The formula relates column efficiency (number of plates) to peak width at half height, with retention time as a scaling factor.
Details: Peak width at half height is a key parameter in chromatography for assessing column performance, resolution, and separation efficiency.
Tips: Enter the number of theoretical plates (typically ranges from 1000 to 100,000) and retention time in seconds. All values must be > 0.
Q1: What does the number of theoretical plates represent?
A: It's a measure of column efficiency - higher numbers indicate better separation and narrower peaks.
Q2: How is retention time measured?
A: Retention time is the time between injection and when the peak maximum elutes from the column.
Q3: Why is peak width important?
A: Narrower peaks (smaller width) indicate better separation and higher column efficiency.
Q4: What's a typical number of theoretical plates?
A: For HPLC columns, 10,000-20,000 plates is common; for GC columns, it can be much higher.
Q5: Can I use this for any chromatographic technique?
A: Yes, the formula applies to HPLC, GC, and other chromatographic methods.