Formula Used:
From: | To: |
Definition: The Half of Average Width of Peak is the addition of the width of the number of peaks divided by twice the total number of peaks.
Purpose: This measurement is important in chromatography to determine peak resolution and column efficiency.
The calculator uses the formula:
Where:
Explanation: The formula relates the peak width at half height to the resolution and retention time difference between two peaks.
Details: Understanding peak width helps in assessing column performance, optimizing separation conditions, and ensuring accurate quantitative analysis.
Tips: Enter the change in retention time (difference between two peaks' retention times) and the resolution between them. Both values must be positive numbers.
Q1: What does the 0.589 constant represent?
A: This is a conversion factor that relates the peak width at half height to the baseline width in Gaussian peaks.
Q2: How is resolution (R) determined?
A: Resolution is calculated from chromatographic data using the retention times and peak widths of two adjacent peaks.
Q3: What affects peak width in chromatography?
A: Peak width is influenced by column efficiency, flow rate, temperature, and sample interactions with the stationary phase.
Q4: Why measure at half height?
A: Measuring at half peak height provides a consistent reference point that's less affected by baseline noise.
Q5: How does peak width relate to column efficiency?
A: Narrower peaks (smaller width) generally indicate higher column efficiency and better separation capability.