Inhibitor Concentration Formula:
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Definition: This calculator determines the concentration of inhibitor needed based on the enzyme substrate modifying factor and the enzyme-substrate dissociation constant.
Purpose: It helps biochemists and researchers quantify the amount of inhibitor required for enzyme inhibition studies.
The calculator uses the formula:
Where:
Explanation: The modifying factor minus one is multiplied by the dissociation constant to determine the required inhibitor concentration.
Details: Accurate inhibitor concentration is crucial for studying enzyme kinetics, drug development, and understanding biochemical pathways.
Tips: Enter the enzyme substrate modifying factor (α') and the enzyme-substrate dissociation constant (Ki'). All values must be > 0.
Q1: What is the enzyme substrate modifying factor?
A: It's a factor that describes how an inhibitor affects the apparent dissociation constant of the enzyme-substrate complex.
Q2: How do I determine the enzyme-substrate dissociation constant?
A: This is typically determined experimentally through kinetic studies of the enzyme-substrate interaction.
Q3: What units should I use for the dissociation constant?
A: The calculator uses mol/m³, but you can convert from other units (like Molar) as needed.
Q4: What does a modifying factor of 1 mean?
A: A factor of 1 indicates no effect on the enzyme-substrate complex, resulting in zero inhibitor concentration.
Q5: Can this be used for competitive inhibition?
A: This formula is specifically for uncompetitive inhibition where the inhibitor binds only to the enzyme-substrate complex.