1. What is Inhibitor Concentration given Enzyme Substrate Modifying Factor?

Definition: This calculator determines the concentration of inhibitor needed based on the enzyme substrate modifying factor and the enzyme-substrate dissociation constant.

Purpose: It helps biochemists and researchers quantify the amount of inhibitor required for enzyme inhibition studies.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( I \) — Inhibitor concentration (mol/m³)
• \( \alpha' \) — Enzyme substrate modifying factor
• \( K_i' \) — Enzyme-substrate dissociation constant (mol/m³)

Explanation: The modifying factor minus one is multiplied by the dissociation constant to determine the required inhibitor concentration.

3. Importance of Inhibitor Concentration Calculation

Details: Accurate inhibitor concentration is crucial for studying enzyme kinetics, drug development, and understanding biochemical pathways.

4. Using the Calculator

Tips: Enter the enzyme substrate modifying factor (α') and the enzyme-substrate dissociation constant (K_i'). All values must be > 0.

5. Frequently Asked Questions (FAQ)

Q1: What is the enzyme substrate modifying factor?
A: It's a factor that describes how an inhibitor affects the apparent dissociation constant of the enzyme-substrate complex.

Q2: How do I determine the enzyme-substrate dissociation constant?
A: This is typically determined experimentally through kinetic studies of the enzyme-substrate interaction.

Q3: What units should I use for the dissociation constant?
A: The calculator uses mol/m³, but you can convert from other units (like Molar) as needed.

Q4: What does a modifying factor of 1 mean?
A: A factor of 1 indicates no effect on the enzyme-substrate complex, resulting in zero inhibitor concentration.

Q5: Can this be used for competitive inhibition?
A: This formula is specifically for uncompetitive inhibition where the inhibitor binds only to the enzyme-substrate complex.