Inhibitor Concentration Formula:
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Definition: This calculator determines the concentration of an uncompetitive inhibitor based on enzyme-substrate dissociation constant and complex concentrations.
Purpose: It helps biochemists and researchers quantify inhibitor concentration in enzyme kinetics studies involving uncompetitive inhibition.
The calculator uses the formula:
Where:
Explanation: The formula calculates inhibitor concentration by relating the dissociation constant to the concentrations of enzyme complexes.
Details: Accurate determination of inhibitor concentration is crucial for understanding enzyme inhibition mechanisms and designing effective inhibitors for therapeutic applications.
Tips: Enter the enzyme-substrate dissociation constant (Ki'), enzyme-substrate-inhibitor complex concentration ([ESI]), and enzyme-substrate complex concentration ([ES]). All values must be > 0.
Q1: What is uncompetitive inhibition?
A: Uncompetitive inhibition occurs when an inhibitor binds only to the enzyme-substrate complex, not to the free enzyme.
Q2: How is Ki' different from Ki?
A: Ki' is the dissociation constant specific to uncompetitive inhibition, representing the affinity of the inhibitor for the ES complex.
Q3: What units should I use for concentrations?
A: The calculator uses mol/m³, but you can convert from other units (1 M = 1000 mol/m³).
Q4: Can this calculator be used for other inhibition types?
A: No, this specific formula applies only to uncompetitive inhibition scenarios.
Q5: What if I get negative results?
A: Negative results are not physically meaningful. Check your input values and ensure all concentrations are positive.