Inhibitor Concentration in Presence of Uncompetitive Inhibitor Calculator

Inhibitor Concentration Formula:

\[ I = \frac{Ki' \times [ESI]}{[ES]} \]

Enzyme Substrate Dissociation Constant (Ki'):

mol/m³

Enzyme Substrate Inhibitor Complex Concentration ([ESI]):

mol/m³

Enzyme Substrate Complex Concentration ([ES]):

mol/m³

Inhibitor Concentration (mol/m³):

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1. What is Inhibitor Concentration in Presence of Uncompetitive Inhibitor?

Definition: This calculator determines the concentration of an uncompetitive inhibitor based on enzyme-substrate dissociation constant and complex concentrations.

Purpose: It helps biochemists and researchers quantify inhibitor concentration in enzyme kinetics studies involving uncompetitive inhibition.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ I = \frac{Ki' \times [ESI]}{[ES]} \]

Where:

\( I \) — Inhibitor concentration (mol/m³)
\( Ki' \) — Enzyme-substrate dissociation constant (mol/m³)
\( [ESI] \) — Enzyme-substrate-inhibitor complex concentration (mol/m³)
\( [ES] \) — Enzyme-substrate complex concentration (mol/m³)

Explanation: The formula calculates inhibitor concentration by relating the dissociation constant to the concentrations of enzyme complexes.

3. Importance of Inhibitor Concentration Calculation

Details: Accurate determination of inhibitor concentration is crucial for understanding enzyme inhibition mechanisms and designing effective inhibitors for therapeutic applications.

4. Using the Calculator

Tips: Enter the enzyme-substrate dissociation constant (Ki'), enzyme-substrate-inhibitor complex concentration ([ESI]), and enzyme-substrate complex concentration ([ES]). All values must be > 0.

5. Frequently Asked Questions (FAQ)

Q1: What is uncompetitive inhibition?
A: Uncompetitive inhibition occurs when an inhibitor binds only to the enzyme-substrate complex, not to the free enzyme.

Q2: How is Ki' different from Ki?
A: Ki' is the dissociation constant specific to uncompetitive inhibition, representing the affinity of the inhibitor for the ES complex.

Q3: What units should I use for concentrations?
A: The calculator uses mol/m³, but you can convert from other units (1 M = 1000 mol/m³).

Q4: Can this calculator be used for other inhibition types?
A: No, this specific formula applies only to uncompetitive inhibition scenarios.

Q5: What if I get negative results?
A: Negative results are not physically meaningful. Check your input values and ensure all concentrations are positive.