Enzyme Conservation Formula:
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Definition: The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Purpose: This calculator determines the total enzyme concentration based on the enzyme conservation law in biochemical reactions.
The calculator uses the formula:
Where:
Explanation: The total enzyme concentration equals the sum of free enzyme and enzyme bound in complexes with substrate.
Details: This fundamental principle in enzyme kinetics ensures mass balance in enzymatic reactions and is crucial for understanding reaction mechanisms.
Tips: Enter the free enzyme concentration and enzyme-substrate complex concentration in mol/m³. Both values must be ≥ 0.
Q1: What units should I use for the concentrations?
A: The calculator uses mol/m³ (SI units), but you can convert from other units like M (mol/L) by multiplying by 1000.
Q2: Does this apply to all enzyme reactions?
A: Yes, the enzyme conservation law applies to all single-substrate enzymatic reactions.
Q3: How do I measure [ES] experimentally?
A: [ES] can be estimated using spectroscopic methods or by measuring reaction rates under different conditions.
Q4: What if I have multiple substrates?
A: The principle still applies but becomes more complex with additional terms for each enzyme-substrate complex.
Q5: Can [E₀] change during a reaction?
A: No, [E₀] is constant - it's the total enzyme present at the start of the reaction.