Enzyme Concentration Formula:
From: | To: |
Definition: This calculator determines the initial concentration of enzyme needed when substrate concentration is much higher than the Michaelis constant (Km).
Purpose: It helps biochemists and enzymologists estimate enzyme requirements for reactions operating at maximum velocity.
The calculator uses the formula:
Where:
Explanation: The formula calculates the enzyme concentration required to achieve a specific maximum reaction rate given the enzyme's turnover number.
Details: Accurate enzyme concentration estimation ensures proper reaction rates, cost-effective enzyme usage, and valid experimental conditions.
Tips: Enter the maximum rate (Vmax) in mol/m³·s and catalytic rate constant (kcat) in s⁻¹. Both values must be > 0.
Q1: When is this calculation valid?
A: Only when substrate concentration [S] is much greater than the Michaelis constant (Km), i.e., [S] >> Km.
Q2: How do I determine Vmax?
A: Vmax can be determined experimentally from Lineweaver-Burk plots or other kinetic analyses.
Q3: What affects kcat values?
A: kcat depends on enzyme type, temperature, pH, and other reaction conditions.
Q4: Can I use different concentration units?
A: Yes, but all units must be consistent (e.g., if using mM for concentration, use mM/s for Vmax).
Q5: Does this account for enzyme inhibition?
A: No, this calculation assumes ideal conditions without inhibitors.