Lifetime Ratio Formula:
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Definition: The Lifetime Ratio is the ratio of singlet state lifetime without quencher to singlet state lifetime with quencher of a given system.
Purpose: It helps in studying fluorescence quenching and understanding molecular interactions in photochemistry.
The calculator uses the formula:
Where:
Explanation: The formula calculates how much the lifetime of the excited state is reduced in the presence of a quencher.
Details: This measurement is crucial in fluorescence spectroscopy for understanding quenching mechanisms and molecular interactions.
Tips: Enter the quenching constant in Hz, singlet lifetime in seconds, and quencher concentration. All values must be > 0.
Q1: What is quenching in fluorescence?
A: Quenching refers to any process that decreases the fluorescence intensity of a given substance.
Q2: What's a typical quenching constant value?
A: The value varies widely depending on the system, typically ranging from 106 to 1010 M-1s-1 for diffusion-controlled quenching.
Q3: How is singlet lifetime measured?
A: It's typically measured using time-resolved fluorescence spectroscopy techniques.
Q4: What affects the lifetime ratio?
A: Temperature, solvent viscosity, and the nature of the fluorophore-quencher interaction all affect the ratio.
Q5: What does a high lifetime ratio indicate?
A: A high ratio indicates strong quenching, meaning the quencher effectively reduces the excited state lifetime.