Mass of Analyte Formula:
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Definition: This calculator determines the mass of the first analyte based on the mass of a second analyte and the ratio of their column radii squared.
Purpose: It's used in chromatography and analytical chemistry to scale analyte masses when changing column dimensions.
The calculator uses the formula:
Where:
Explanation: The mass scales with the square of the column radius ratio, maintaining constant linear velocity and pressure.
Details: Proper scaling ensures equivalent chromatographic performance when transferring methods between columns of different diameters.
Tips: Enter the known mass of the second analyte and both column radii. All values must be > 0.
Q1: Why does mass scale with radius squared?
A: Because column cross-sectional area (which determines sample capacity) increases with the square of the radius.
Q2: Does this account for column length differences?
A: No, this assumes equal column lengths. For different lengths, additional scaling is needed.
Q3: What units should I use?
A: Consistent units are required (kg for mass, m for radius), but any consistent units will work.
Q4: When would I use this calculation?
A: When scaling up preparative chromatography or transferring analytical methods between different column sizes.
Q5: Does this work for all analyte types?
A: Generally yes, but for very large molecules or extreme conditions, additional factors may need consideration.