Formula Used:
From: | To: |
Definition: This calculator determines the time taken by the mobile phase to move over the length of the column in chromatography.
Purpose: It helps in chromatographic analysis by calculating the unretained solute travel time, which is important for understanding column behavior.
The calculator uses the formula:
Where:
Explanation: The adjusted retention time is subtracted from the total retention time to get the mobile phase travel time.
Details: Knowing the mobile phase travel time helps in method development, column efficiency calculations, and system suitability tests in chromatography.
Tips: Enter the retention time and adjusted retention time in seconds. Both values must be positive numbers, and retention time should be greater than adjusted retention time.
Q1: What is the difference between retention time and adjusted retention time?
A: Retention time is the total time a solute spends in the column, while adjusted retention time is the retention time minus the time taken by unretained components.
Q2: Why is mobile phase travel time important?
A: It helps determine the void volume of the column and is used in calculating retention factors and column efficiency.
Q3: What units should I use for the inputs?
A: The calculator uses seconds, but any consistent time unit can be used as long as both inputs are in the same units.
Q4: Can the adjusted retention time be greater than the retention time?
A: No, the adjusted retention time is always less than the total retention time as it's derived by subtracting the mobile phase travel time.
Q5: How is this related to chromatographic resolution?
A: Mobile phase travel time is used in calculating retention factors, which are important parameters in resolution equations.