1. What is an Original Test Portion Calculator?

Definition: This calculator determines the original portion of a solution needed to achieve a desired final volume at a specific dilution factor.

Purpose: It helps laboratory technicians and researchers prepare accurate dilutions for experiments and analyses.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( V_o \) — Original portion (cubic meters)
• \( V_f \) — Final volume (cubic meters)
• \( D_f \) — Dilution factor (dimensionless)

Explanation: The final volume is divided by the dilution factor to determine how much of the original solution is needed.

3. Importance of Accurate Dilution Calculations

Details: Proper dilution calculations ensure experimental accuracy, reagent conservation, and reproducible results in scientific work.

4. Using the Calculator

Tips: Enter the desired final volume and the dilution factor. Both values must be positive numbers (dilution factor must be > 0).

5. Frequently Asked Questions (FAQ)

Q1: What is a dilution factor?
A: The dilution factor is the ratio of the aliquot volume to the final volume of a solution. It represents how much the original solution is being diluted.

Q2: How do I determine the dilution factor?
A: The dilution factor is typically specified in your experimental protocol or can be calculated as final volume divided by aliquot volume.

Q3: Can I use different units for volume?
A: Yes, as long as you're consistent. The calculator uses m³ but you can use liters or mL as long as all volume inputs use the same unit.

Q4: What if my dilution factor is less than 1?
A: A dilution factor < 1 would mean you're concentrating rather than diluting, which is uncommon in most applications.

Q5: How precise should my measurements be?
A: For most laboratory work, measurements should be precise to at least 3 significant figures for reliable results.