1. What is Partition Coefficient of Protein?

Definition: The partition coefficient is a measure of the distribution of a protein between two immiscible phases in aqueous two-phase extraction.

Purpose: It helps biochemists and researchers understand protein partitioning behavior in different phases during extraction processes.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( K_{part} \) — Partition coefficient (dimensionless)
• \( OD_{top\ phase} \) — Optical density of top phase extracts
• \( OD_{bottom\ phase} \) — Optical density of bottom phase extracts

Explanation: The optical density measurements from both phases are used to calculate the distribution ratio of the protein between them.

3. Importance of Partition Coefficient

Details: The partition coefficient indicates protein preference for one phase over another, which is crucial for designing efficient extraction and purification processes.

4. Using the Calculator

Tips: Enter the optical density measurements from both phases. Both values must be > 0. The result is dimensionless.

5. Frequently Asked Questions (FAQ)

Q1: What does a K_part > 1 indicate?
A: A value greater than 1 means the protein preferentially partitions into the top phase.

Q2: What does a K_part < 1 indicate?
A: A value less than 1 means the protein preferentially partitions into the bottom phase.

Q3: What's a typical range for protein partition coefficients?
A: Most proteins have partition coefficients between 0.1 and 10 in aqueous two-phase systems.

Q4: How do I measure optical density?
A: Optical density is typically measured using a spectrophotometer at an appropriate wavelength (often 280nm for proteins).

Q5: Does temperature affect the partition coefficient?
A: Yes, temperature can significantly affect protein partitioning behavior in two-phase systems.