Partition Coefficient Formula:
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Definition: The partition coefficient is a measure of the distribution of a protein between two immiscible phases in aqueous two-phase extraction.
Purpose: It helps biochemists and researchers understand protein partitioning behavior in different phases during extraction processes.
The calculator uses the formula:
Where:
Explanation: The optical density measurements from both phases are used to calculate the distribution ratio of the protein between them.
Details: The partition coefficient indicates protein preference for one phase over another, which is crucial for designing efficient extraction and purification processes.
Tips: Enter the optical density measurements from both phases. Both values must be > 0. The result is dimensionless.
Q1: What does a Kpart > 1 indicate?
A: A value greater than 1 means the protein preferentially partitions into the top phase.
Q2: What does a Kpart < 1 indicate?
A: A value less than 1 means the protein preferentially partitions into the bottom phase.
Q3: What's a typical range for protein partition coefficients?
A: Most proteins have partition coefficients between 0.1 and 10 in aqueous two-phase systems.
Q4: How do I measure optical density?
A: Optical density is typically measured using a spectrophotometer at an appropriate wavelength (often 280nm for proteins).
Q5: Does temperature affect the partition coefficient?
A: Yes, temperature can significantly affect protein partitioning behavior in two-phase systems.