Protein Recovery Formula:
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Definition: Protein recovery percentage measures the efficiency of protein purification processes by comparing the final protein concentration to the initial concentration.
Purpose: It helps biochemists and researchers evaluate the effectiveness of protein purification techniques like salting out.
The calculator uses the formula:
Where:
Explanation: The ratio of final to initial concentration shows what percentage of the original protein was successfully recovered.
Details: High recovery percentages indicate efficient purification methods, while low percentages may suggest protein loss during processing.
Tips: Enter both concentration values in kg/m³. Initial concentration must be greater than zero.
Q1: What's considered a good recovery percentage?
A: Typically 70-90% is good, but this varies by method and protein type.
Q2: Why might recovery be over 100%?
A: This could indicate measurement errors or concentration of the protein during purification.
Q3: How do I measure protein concentrations?
A: Common methods include Bradford assay, UV absorbance at 280nm, or BCA assay.
Q4: Does this account for protein purity?
A: No, this only measures quantity recovered, not purity. Use SDS-PAGE for purity analysis.
Q5: What affects protein recovery rates?
A: Factors include precipitation efficiency, centrifugation parameters, and solubility characteristics.