Resolution Formula:
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Definition: This calculator determines the resolution power of a column based on the number of theoretical plates and separation factor.
Purpose: It helps chromatographers evaluate column performance and separation effectiveness in analytical chemistry.
The calculator uses the formula:
Where:
Explanation: The square root of theoretical plates divided by 4 gives the column efficiency component, which is then multiplied by the separation factor minus 1.
Details: Higher resolution values indicate better separation between peaks in chromatography. Values above 1.5 typically indicate baseline separation.
Tips: Enter the number of theoretical plates (N) and separation factor (β). Both values must be > 0.
Q1: What is a good resolution value?
A: Resolution ≥ 1.5 is generally considered good for baseline separation of peaks.
Q2: How do I determine the number of theoretical plates?
A: N can be calculated from chromatographic data using N = 16(tR/W)^2 where tR is retention time and W is peak width.
Q3: What affects the separation factor?
A: The separation factor depends on column chemistry, mobile phase composition, temperature, and analyte properties.
Q4: Can resolution be less than 1?
A: Yes, values < 1 indicate poor separation where peaks overlap significantly.
Q5: How can I improve resolution?
A: Increase N (longer column, smaller particles) or β (change stationary/mobile phase).