1. What is Resolution of Two Peaks?

Definition: Resolution is defined as the resolving power of the column to separate two peaks in chromatography.

Purpose: It helps analytical chemists determine how well a chromatographic system can distinguish between two compounds.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( R \) — Resolution (unitless)
• \( \Delta t_r \) — Change in retention time (seconds)
• \( w_{av} \) — Average width of peaks (seconds)

Explanation: The difference in retention times is divided by the average peak width to determine the resolution between two peaks.

3. Importance of Resolution Calculation

Details: Proper resolution ensures accurate compound identification and quantification in chromatographic analysis.

4. Using the Calculator

Tips: Enter the change in retention time and average peak width in seconds. Both values must be > 0.

5. Frequently Asked Questions (FAQ)

Q1: What is considered good resolution?
A: A resolution of 1.5 or higher is generally considered baseline separation between peaks.

Q2: How do I measure retention time difference?
A: Subtract the retention time of the first peak from the second peak in your chromatogram.

Q3: How is average peak width calculated?
A: Add the widths at baseline of both peaks and divide by two.

Q4: What affects resolution in chromatography?
A: Column efficiency, selectivity, mobile phase composition, temperature, and flow rate.

Q5: Can resolution be less than 1?
A: Yes, but peaks with R < 1 are considered poorly resolved or overlapping.