Retention Volume Formula:
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Definition: This calculator determines the retention volume based on the capacity factor and unretained mobile phase volume in chromatography.
Purpose: It helps analytical chemists and researchers calculate the volume required to elute a solute from a chromatographic column.
The calculator uses the formula:
Where:
Explanation: The retention volume is calculated by adding 1 to the capacity factor and multiplying by the unretained mobile phase volume.
Details: Accurate retention volume calculation is crucial for method development in chromatography, ensuring proper separation of compounds.
Tips: Enter the capacity factor (k') and unretained mobile phase volume (Vm). The mobile phase volume must be > 0, while capacity factor can be ≥ 0.
Q1: What is the capacity factor (k')?
A: The capacity factor is a measure of how long a compound is retained on the column relative to an unretained compound.
Q2: How do I determine the unretained mobile phase volume?
A: It's typically measured as the volume required to elute a non-retained compound (dead volume) through the column.
Q3: What units should I use for the mobile phase volume?
A: The calculator uses cubic meters (m³), but you can convert from mL (1 mL = 1×10⁻⁶ m³) if needed.
Q4: What does a capacity factor of 0 mean?
A: A k' of 0 means the compound elutes with the void volume (unretained), making retention volume equal to Vm.
Q5: How does this relate to retention time?
A: Retention volume can be converted to retention time by dividing by the flow rate of the mobile phase.