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Reverse Rate Constant given Forward and Catalytic Rate Constants Calculator

Reverse Rate Constant Formula:

\[ k_r = \frac{(k_f \times E \times S)}{ES} - k_{cat} \]

s-1
mol/m³
mol/m³
mol/m³
s-1

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1. What is Reverse Rate Constant?

Definition: The Reverse Rate Constant (kr) is the rate constant for the backward reaction in enzyme kinetics.

Purpose: It helps quantify how quickly the enzyme-substrate complex dissociates back into free enzyme and substrate.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ k_r = \frac{(k_f \times E \times S)}{ES} - k_{cat} \]

Where:

Explanation: The formula calculates the reverse rate constant by accounting for the forward reaction and catalytic conversion.

3. Importance of Reverse Rate Constant

Details: Understanding kr is crucial for determining enzyme efficiency, reaction equilibrium, and designing enzyme inhibitors.

4. Using the Calculator

Tips: Enter all required values in appropriate units. All concentrations must be positive, and rate constants non-negative.

5. Frequently Asked Questions (FAQ)

Q1: What are typical values for kr?
A: Values vary widely but often range from 10⁻³ to 10³ s⁻¹ depending on the enzyme and substrate.

Q2: How does kr relate to enzyme affinity?
A: Higher kr values indicate weaker enzyme-substrate binding (lower affinity).

Q3: Can kr be negative?
A: No, if you get a negative result, check your input values as this indicates an error.

Q4: What's the difference between kr and kcat?
A: kr describes substrate release, while kcat describes product formation.

Q5: How is this used in drug design?
A: Drugs often aim to modify kr to either enhance or inhibit enzyme activity.

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