Reverse Rate Constant Formula:
From: | To: |
Definition: This calculator determines the reverse rate constant (kr) in enzyme kinetics based on the Michaelis constant (KM), forward rate constant (kf), and catalytic rate constant (kcat).
Purpose: It helps biochemists and enzymologists analyze enzyme kinetics and understand reaction mechanisms.
The calculator uses the formula:
Where:
Explanation: The formula calculates the reverse rate constant from fundamental enzyme kinetic parameters.
Details: The reverse rate constant is crucial for understanding enzyme mechanisms, determining reaction equilibrium, and analyzing inhibitor effects.
Tips: Enter the Michaelis constant (KM), forward rate constant (kf), and catalytic rate constant (kcat). All values must be ≥ 0.
Q1: What is the Michaelis constant?
A: The substrate concentration at which the reaction rate is half of Vmax.
Q2: How is the forward rate constant determined?
A: Typically measured experimentally or calculated from kinetic studies.
Q3: What if I get a negative result?
A: Negative values suggest the catalytic rate exceeds the product (KM × kf), which may indicate measurement errors.
Q4: What units should I use?
A: Maintain consistent units (mol/m³ for KM, s⁻¹ for rate constants).
Q5: How accurate is this calculation?
A: Accuracy depends on the precision of your input parameters.