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Reverse Rate Constant given Michaelis Constant Calculator

Reverse Rate Constant Formula:

\[ k_r = (K_M \times k_f) - k_{cat} \]

mol/m³
s⁻¹
s⁻¹
mol/m³·s

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1. What is Reverse Rate Constant given Michaelis Constant?

Definition: This calculator determines the reverse rate constant (kr) in enzyme kinetics based on the Michaelis constant (KM), forward rate constant (kf), and catalytic rate constant (kcat).

Purpose: It helps biochemists and enzymologists analyze enzyme kinetics and understand reaction mechanisms.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ k_r = (K_M \times k_f) - k_{cat} \]

Where:

Explanation: The formula calculates the reverse rate constant from fundamental enzyme kinetic parameters.

3. Importance in Enzyme Kinetics

Details: The reverse rate constant is crucial for understanding enzyme mechanisms, determining reaction equilibrium, and analyzing inhibitor effects.

4. Using the Calculator

Tips: Enter the Michaelis constant (KM), forward rate constant (kf), and catalytic rate constant (kcat). All values must be ≥ 0.

5. Frequently Asked Questions (FAQ)

Q1: What is the Michaelis constant?
A: The substrate concentration at which the reaction rate is half of Vmax.

Q2: How is the forward rate constant determined?
A: Typically measured experimentally or calculated from kinetic studies.

Q3: What if I get a negative result?
A: Negative values suggest the catalytic rate exceeds the product (KM × kf), which may indicate measurement errors.

Q4: What units should I use?
A: Maintain consistent units (mol/m³ for KM, s⁻¹ for rate constants).

Q5: How accurate is this calculation?
A: Accuracy depends on the precision of your input parameters.

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