Reverse Rate Constant Formula:
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Definition: The reverse rate constant (kr) quantifies the rate at which the enzyme-substrate complex dissociates back into free enzyme and substrate.
Purpose: This calculator helps biochemists and enzymologists determine the reverse rate constant in Michaelis-Menten kinetics.
The calculator uses the formula:
Where:
Explanation: The formula calculates how quickly the enzyme-substrate complex breaks down based on reaction parameters.
Details: Understanding kr is crucial for modeling enzyme kinetics, determining reaction equilibrium, and designing enzyme inhibitors.
Tips: Enter all positive values with [E0] > ES. Typical units shown, but ensure consistency in your measurements.
Q1: What's the relationship between kr and enzyme efficiency?
A: Lower kr values generally indicate more stable enzyme-substrate complexes and potentially more efficient catalysis.
Q2: How do I measure the forward rate constant (kf)?
A: kf is typically determined experimentally using stopped-flow techniques or other rapid kinetic methods.
Q3: What if my [E0] is less than ES?
A: This is impossible physically - check your measurements as ES cannot exceed [E0].
Q4: How does temperature affect kr?
A: Like most rate constants, kr typically increases with temperature following Arrhenius equation behavior.
Q5: Can I use this for non-enzymatic reactions?
A: The formula applies to any reversible reaction following similar kinetics, not just enzymatic ones.