Standard Deviation Formula:
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Definition: This calculator computes the standard deviation based on the plate height and length of a chromatographic column.
Purpose: It helps in chromatography analysis to understand the dispersion of particles in the column.
The calculator uses the formula:
Where:
Explanation: The standard deviation is calculated as the square root of the product of plate height and column length.
Details: This measurement is crucial for understanding the efficiency and resolution of chromatographic separations.
Tips: Enter the plate height and column length in meters. Both values must be > 0.
Q1: What is plate height in chromatography?
A: Plate height (H) is a measure of column efficiency, representing the height equivalent to one theoretical plate.
Q2: How does column length affect standard deviation?
A: Longer columns typically result in greater standard deviation values, indicating broader peaks.
Q3: What are typical values for plate height?
A: Plate height values typically range from micrometers to millimeters depending on column packing and particle size.
Q4: How is this standard deviation related to peak width?
A: The standard deviation is directly related to peak width at the base (4σ) and at half height (2.35σ).
Q5: Can this be used for other types of columns?
A: Yes, the formula applies to various chromatographic columns including GC, HPLC, and SEC columns.