Stern Volmner Constant Formula:
From: | To: |
Definition: The Stern Volmner Constant is the bimolecular rate constant of the fluorescence quenching process due to a short-range interaction of species.
Purpose: It helps quantify the efficiency of fluorescence quenching by a quencher molecule.
The calculator uses the formula:
Where:
Explanation: The ratio of intensities minus one is divided by the quencher concentration to determine the quenching efficiency.
Details: This constant is crucial in photochemistry for understanding molecular interactions and energy transfer processes.
Tips: Enter the intensity values in W/m² and quencher concentration. All values must be > 0.
Q1: What does a higher Stern Volmner Constant indicate?
A: A higher value indicates more efficient quenching, meaning the quencher is more effective at reducing fluorescence.
Q2: What are typical units for Stern Volmner Constant?
A: The constant is typically expressed in M⁻¹ (per molar concentration).
Q3: When would I get a negative Stern Volmner Constant?
A: Negative values are physically meaningless and usually indicate measurement errors or input mistakes.
Q4: How do I measure fluorescence intensity?
A: Use a fluorometer or spectrofluorometer to measure the emission intensity at the appropriate wavelength.
Q5: What factors affect the Stern Volmner Constant?
A: Temperature, solvent viscosity, and the nature of the fluorophore-quencher interaction all affect the constant.