Substrate Concentration Formula:
From: | To: |
Definition: Substrate concentration is the number of moles of substrate per liter solution in an enzyme-catalyzed reaction.
Purpose: This calculator helps determine the substrate concentration needed for a given initial reaction rate in enzyme kinetics.
The calculator uses the formula:
Where:
Explanation: The formula calculates substrate concentration based on enzyme kinetics parameters.
Details: Proper substrate concentration is crucial for enzyme activity studies, drug development, and biochemical research.
Tips: Enter all required parameters. Ensure denominator (kcat×[E0] - V0) is positive.
Q1: What is the typical range for KD?
A: KD values vary widely but often range from 10⁻⁶ to 10⁻³ mol/m³ for enzyme-substrate complexes.
Q2: How do I measure initial reaction rate V0?
A: V0 is typically measured experimentally as the slope of product concentration vs time at t=0.
Q3: What if my calculation gives a negative value?
A: This means (kcat×[E0]) ≤ V0, which is physically impossible. Check your input values.
Q4: What units should I use?
A: Consistent units are required (mol/m³ for concentrations, s⁻¹ for rate constants).
Q5: Can I use molar (M) instead of mol/m³?
A: Yes, but convert all concentrations (1 M = 1000 mol/m³).