Substrate Concentration Formula:
From: | To: |
Definition: Substrate concentration is the number of moles of substrate per liter of solution in an enzyme-catalyzed reaction.
Purpose: This calculator helps determine the substrate concentration based on enzyme kinetics parameters.
The calculator uses the formula:
Where:
Explanation: The formula calculates substrate concentration from enzyme kinetics parameters, accounting for both the forward and reverse reactions.
Details: Knowing substrate concentration is crucial for understanding reaction rates, enzyme efficiency, and designing biochemical experiments.
Tips: Enter all required rate constants and concentrations. Forward rate constant and catalyst concentration must be greater than zero.
Q1: What are typical units for these parameters?
A: Rate constants typically use 1/s or mol/(m³·s), while concentrations use mol/m³.
Q2: How do I determine the rate constants experimentally?
A: Rate constants are typically determined through kinetic experiments measuring reaction rates at different substrate concentrations.
Q3: What's the difference between kcat and kf?
A: kcat is the catalytic rate constant for product formation, while kf is the forward rate constant for ES complex formation.
Q4: Can this be used for non-enzymatic catalysts?
A: Yes, the same principles apply to any catalytic system following Michaelis-Menten kinetics.
Q5: What if my enzyme concentration is very low?
A: The calculator remains valid, but experimental measurements at very low [E] may have higher relative errors.