Substrate Concentration Formula:
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Definition: The Substrate Concentration is the number of moles of substrate per liter solution in a chemical reaction.
Purpose: This calculator helps determine the substrate concentration when you know the dissociation rate constant, initial reaction rate, and maximum rate.
The calculator uses the formula:
Where:
Explanation: The formula calculates substrate concentration based on the relationship between reaction rates and the dissociation constant.
Details: Accurate substrate concentration calculations are crucial for enzyme kinetics studies, biochemical reactions, and pharmaceutical research.
Tips: Enter the dissociation rate constant (KD), initial reaction rate (V0), and maximum rate (Vmax). Note that V0 must be less than Vmax.
Q1: What is the dissociation rate constant (KD)?
A: It's the ratio of the reverse and forward rate constants in a reaction, representing the equilibrium between bound and unbound states.
Q2: How is initial reaction rate (V0) determined?
A: V0 is measured experimentally as the reaction rate at the very beginning of the reaction when product formation is linear.
Q3: What does maximum rate (Vmax) represent?
A: Vmax is the maximum reaction rate achieved when the enzyme is saturated with substrate.
Q4: What units should I use?
A: The calculator uses mol/m³ for concentration and mol/(m³·s) for rates, but other consistent units can be used as long as they're compatible.
Q5: What if I get an error?
A: Ensure V0 is less than Vmax and all values are positive numbers.