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Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration Calculator

Substrate Concentration Formula:

\[ S = \frac{V_0 \times K_M}{k_{cat} \times [E_0]} \]

mol/m³·s
mol/m³
s⁻¹
mol/m³

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1. What is Substrate Concentration when KM ≫ S?

Definition: This calculator determines the substrate concentration when the Michaelis constant is much larger than the substrate concentration, using enzyme kinetics parameters.

Purpose: It helps biochemists and enzymologists understand reaction dynamics when substrate concentration is significantly lower than KM.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ S = \frac{V_0 \times K_M}{k_{cat} \times [E_0]} \]

Where:

Explanation: When KM ≫ S, the reaction follows first-order kinetics with respect to substrate.

3. Importance of This Calculation

Details: Understanding substrate concentration under these conditions is crucial for enzyme characterization, drug development, and metabolic pathway analysis.

4. Using the Calculator

Tips: Enter all positive values for initial reaction rate, Michaelis constant, catalytic rate constant, and initial enzyme concentration.

5. Frequently Asked Questions (FAQ)

Q1: When is KM ≫ S a valid assumption?
A: This applies when substrate concentration is less than 10% of KM, common in early reaction stages or dilute conditions.

Q2: How do I measure V₀ experimentally?
A: Measure product formation or substrate disappearance in the first 5-10% of reaction progress.

Q3: What's a typical kcat value?
A: kcat ranges from 0.1 to 10⁶ s⁻¹ depending on the enzyme and reaction.

Q4: Why is enzyme concentration important?
A: [E₀] affects the maximum reaction rate (Vmax = kcat[E₀]).

Q5: Can I use different concentration units?
A: Yes, but all concentrations must use consistent units (e.g., all in mol/m³ or all in mM).

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