1. What is Substrate Concentration in presence of Uncompetitive Inhibitor?

Definition: This calculator determines the substrate concentration needed to achieve a specific reaction rate when an uncompetitive inhibitor is present.

Purpose: It helps biochemists and enzymologists understand enzyme kinetics in the presence of inhibitors.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( S \) — Substrate concentration (mol/m³)
• \( K_M \) — Michaelis constant (mol/m³)
• \( V_0 \) — Initial reaction rate (mol/m³·s)
• \( V_{max} \) — Maximum reaction rate (mol/m³·s)
• \( \alpha' \) — Enzyme-substrate modifying factor

Explanation: The formula accounts for the effect of uncompetitive inhibition on enzyme kinetics.

3. Importance of This Calculation

Details: Understanding substrate concentration in inhibited reactions is crucial for drug development, metabolic studies, and enzyme characterization.

4. Using the Calculator

Tips: Enter the Michaelis constant, initial reaction rate, maximum rate, and modifying factor. All values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: What is an uncompetitive inhibitor?
A: An inhibitor that binds only to the enzyme-substrate complex, not to the free enzyme.

Q2: How does α' affect the calculation?
A: α' represents how the inhibitor modifies the enzyme-substrate complex's properties.

Q3: What are typical values for K_M?
A: K_M values vary widely but are typically in the micromolar to millimolar range.

Q4: Can V₀ be greater than V_max?
A: No, V₀ must always be less than V_max.

Q5: What if I get a negative result?
A: Check your inputs - V₀ × α' cannot exceed V_max for valid results.