Volume of Mobile Phase given Capacity Factor Calculator

Volume of Mobile Phase Formula:

\[ V_{mobile\ phase} = \frac{V_s \times C_s}{k' \times C_m} \]

Volume of Stationary Phase:

m³

Concentration of Stationary Phase:

mol/m³

Capacity Factor:

Concentration of Mobile Phase:

mol/m³

Volume of Mobile Phase (m³):

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1. What is Volume of Mobile Phase given Capacity Factor?

Definition: This calculator determines the volume of mobile phase required in chromatography based on the stationary phase properties and capacity factor.

Purpose: It helps chemists and chromatographers optimize their separation processes by calculating the appropriate mobile phase volume.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ V_{mobile\ phase} = \frac{V_s \times C_s}{k' \times C_m} \]

Where:

\( V_{mobile\ phase} \) — Volume of mobile phase (m³)
\( V_s \) — Volume of stationary phase (m³)
\( C_s \) — Concentration of stationary phase (mol/m³)
\( k' \) — Capacity factor (dimensionless)
\( C_m \) — Concentration of mobile phase (mol/m³)

Explanation: The formula relates the mobile phase volume to the stationary phase properties and the capacity factor, which measures retention in chromatography.

3. Importance of Mobile Phase Volume Calculation

Details: Accurate calculation ensures proper separation of components, optimal solvent usage, and efficient chromatography runs.

4. Using the Calculator

Tips: Enter the volume of stationary phase (default 0.007 m³), its concentration (default 10000 mol/m³), capacity factor (default 3), and mobile phase concentration (default 6000 mol/m³). All values must be > 0.

5. Frequently Asked Questions (FAQ)

Q1: What is the capacity factor (k')?
A: The capacity factor is a measure of how long a compound is retained on the column relative to the mobile phase.

Q2: How do I determine the volume of stationary phase?
A: This is typically the column volume minus the void volume, often provided by column manufacturers.

Q3: What are typical concentration values?
A: Concentrations vary widely depending on the application, but common ranges are 1000-20000 mol/m³ for both phases.

Q4: Why is mobile phase volume important?
A: It determines elution time, resolution, and solvent consumption in chromatographic separations.

Q5: Can this be used for all chromatography types?
A: The formula applies to partition chromatography (like HPLC) but may need adjustment for other techniques.