Volume of Stationary Phase Formula:
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Definition: The volume of stationary phase is the amount of the motionless part of the chromatography column that interacts with the sample components.
Purpose: This calculation helps in understanding column characteristics and optimizing separation processes in chromatography.
The calculator uses the formula:
Where:
Explanation: The formula relates the volumes and concentrations of both phases through the capacity factor, which describes how long a component is retained by the stationary phase.
Details: Knowing the stationary phase volume helps in method development, column characterization, and predicting retention times in chromatographic separations.
Tips: Enter the volume of mobile phase, concentrations of both phases, and the capacity factor. All values must be positive numbers.
Q1: What is the capacity factor (k')?
A: The capacity factor is a measure of how long a compound is retained by the stationary phase relative to the mobile phase.
Q2: How do I determine the concentration of stationary phase?
A: This is typically provided by the column manufacturer or can be determined experimentally.
Q3: What units should I use for volume?
A: The calculator uses cubic meters (m³), but you can convert from mL (1 mL = 1×10⁻⁶ m³) if needed.
Q4: Can this be used for different chromatography types?
A: Yes, it applies to various chromatography techniques including HPLC, GC, and TLC.
Q5: What's a typical capacity factor range?
A: Ideal separations typically have capacity factors between 1 and 10.