Volume of Stationary Phase given Capacity Factor and Partition Coefficient Calculator

Volume of Stationary Phase Formula:

\[ V_s = \frac{k' \times V_{mobile\ phase}}{K} \]

Volume of Stationary Phase (m³):

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1. What is Volume of Stationary Phase?

Definition: The Volume of Stationary Phase is the amount of motionless part of the chromatography column.

Purpose: This calculator helps determine the volume of the stationary phase based on the capacity factor, mobile phase volume, and partition coefficient.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ V_s = \frac{k' \times V_{mobile\ phase}}{K} \]

Where:

\( V_s \) — Volume of stationary phase (cubic meters)
\( k' \) — Capacity factor (dimensionless)
\( V_{mobile\ phase} \) — Volume of mobile phase (cubic meters)
\( K \) — Partition coefficient (dimensionless)

Explanation: The capacity factor multiplied by mobile phase volume gives a relationship that, when divided by the partition coefficient, yields the stationary phase volume.

3. Importance of Stationary Phase Volume Calculation

Details: Accurate calculation of stationary phase volume is crucial for chromatography column design, separation efficiency, and method development.

4. Using the Calculator

Tips: Enter the capacity factor (k'), volume of mobile phase in cubic meters, and partition coefficient (K). All values must be > 0.

5. Frequently Asked Questions (FAQ)

Q1: What is the capacity factor (k')?
A: The capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor.

Q2: What is the partition coefficient (K)?
A: The partition coefficient is defined as the ratio of the equilibrium concentrations of a dissolved substance in a two-phase system consisting of two largely immiscible solvents.

Q3: How do I determine the volume of mobile phase?
A: The mobile phase volume is typically the void volume of the chromatography column, which can be measured experimentally.

Q4: What units should I use for this calculation?
A: The calculator uses cubic meters for volume, but the capacity factor and partition coefficient are dimensionless.

Q5: Can this be used for different types of chromatography?
A: Yes, this formula applies to various chromatography techniques including HPLC, GC, and column chromatography.