Volume of Stationary Phase Formula:
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Definition: The Volume of Stationary Phase is the amount of motionless part of the chromatography column.
Purpose: This calculator helps determine the volume of the stationary phase based on the capacity factor, mobile phase volume, and partition coefficient.
The calculator uses the formula:
Where:
Explanation: The capacity factor multiplied by mobile phase volume gives a relationship that, when divided by the partition coefficient, yields the stationary phase volume.
Details: Accurate calculation of stationary phase volume is crucial for chromatography column design, separation efficiency, and method development.
Tips: Enter the capacity factor (k'), volume of mobile phase in cubic meters, and partition coefficient (K). All values must be > 0.
Q1: What is the capacity factor (k')?
A: The capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor.
Q2: What is the partition coefficient (K)?
A: The partition coefficient is defined as the ratio of the equilibrium concentrations of a dissolved substance in a two-phase system consisting of two largely immiscible solvents.
Q3: How do I determine the volume of mobile phase?
A: The mobile phase volume is typically the void volume of the chromatography column, which can be measured experimentally.
Q4: What units should I use for this calculation?
A: The calculator uses cubic meters for volume, but the capacity factor and partition coefficient are dimensionless.
Q5: Can this be used for different types of chromatography?
A: Yes, this formula applies to various chromatography techniques including HPLC, GC, and column chromatography.