Width of Peak Formula:
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Definition: This calculator determines the width of a chromatographic peak based on the column length and number of theoretical plates.
Purpose: It helps in chromatographic analysis to understand peak broadening and column efficiency.
The calculator uses the formula:
Where:
Explanation: The peak width is directly proportional to column length and inversely proportional to the square root of the number of theoretical plates.
Details: Understanding peak width helps in assessing column efficiency, resolution between peaks, and optimizing separation conditions.
Tips: Enter the column length in meters and the number of theoretical plates. Both values must be > 0.
Q1: What are theoretical plates in chromatography?
A: Theoretical plates represent the efficiency of a chromatographic column, with higher numbers indicating better separation efficiency.
Q2: Why does peak width matter?
A: Narrower peaks generally mean better resolution between components and more efficient separations.
Q3: How is column length related to peak width?
A: Longer columns typically produce broader peaks due to increased longitudinal diffusion.
Q4: What's a typical number of theoretical plates?
A: This varies by column type, but good columns typically have 10,000-20,000 plates per meter.
Q5: Can I use this for different chromatographic techniques?
A: Yes, this formula applies to GC, HPLC, and other chromatographic methods.