Protein Yield Formula:
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Definition: This calculator estimates the yield of protein extracted using aqueous two-phase extraction.
Purpose: It helps researchers and biotechnologists determine the efficiency of protein extraction processes.
The calculator uses the formula:
Where:
Explanation: The formula compares the protein content in the top phase to that in the bottom phase to determine extraction efficiency.
Details: Accurate yield calculation helps optimize extraction processes, compare different methods, and ensure reproducibility in protein purification.
Tips: Enter the volumes and optical densities for both phases. All values must be > 0. Volumes are typically in m³ (1 mL = 1×10⁻⁶ m³).
Q1: What is aqueous two-phase extraction?
A: It's a liquid-liquid extraction method using two immiscible aqueous phases to separate biomolecules like proteins.
Q2: How do I measure optical density?
A: Optical density is typically measured using a spectrophotometer at 280 nm for proteins.
Q3: What does a yield > 1 mean?
A: A yield > 1 indicates more protein was extracted into the top phase than remained in the bottom phase.
Q4: What factors affect protein yield?
A: Phase composition, pH, temperature, salt concentration, and protein properties all affect yield.
Q5: Can I use different units?
A: Yes, as long as you use consistent units for both volumes (e.g., both in mL or both in m³).