1. What is a Yield of Protein Calculator?

Definition: This calculator estimates the yield of protein extracted using aqueous two-phase extraction.

Purpose: It helps researchers and biotechnologists determine the efficiency of protein extraction processes.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( Y \) — Yield of protein
• \( V_t \) — Volume of top phase (m³)
• \( OD_t \) — Optical density of top phase
• \( V_b \) — Volume of bottom phase (m³)
• \( OD_b \) — Optical density of bottom phase

Explanation: The formula compares the protein content in the top phase to that in the bottom phase to determine extraction efficiency.

3. Importance of Protein Yield Calculation

Details: Accurate yield calculation helps optimize extraction processes, compare different methods, and ensure reproducibility in protein purification.

4. Using the Calculator

Tips: Enter the volumes and optical densities for both phases. All values must be > 0. Volumes are typically in m³ (1 mL = 1×10⁻⁶ m³).

5. Frequently Asked Questions (FAQ)

Q1: What is aqueous two-phase extraction?
A: It's a liquid-liquid extraction method using two immiscible aqueous phases to separate biomolecules like proteins.

Q2: How do I measure optical density?
A: Optical density is typically measured using a spectrophotometer at 280 nm for proteins.

Q3: What does a yield > 1 mean?
A: A yield > 1 indicates more protein was extracted into the top phase than remained in the bottom phase.

Q4: What factors affect protein yield?
A: Phase composition, pH, temperature, salt concentration, and protein properties all affect yield.

Q5: Can I use different units?
A: Yes, as long as you use consistent units for both volumes (e.g., both in mL or both in m³).